Search results for "3D tumor model"

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3D polymeric supports promote the growth and progression of anaplastic thyroid carcinoma.

2020

Abstract Anaplastic thyroid carcinoma (ATC) is a rare and aggressive malignancy that accounts for the majority of deaths from all thyroid cancers. ATC exhibits invasiveness and highly resistance to conventional therapies which include cytotoxic chemotherapy, the combination of BRAF and MEK inhibition and, more recently, immunotherapies, that have shown promising but still limited results. A growing knowledge on ATC tumor biology is needed for developing more effective therapies with significant better survival. Researchers have begun to utilize 3D models to culture cancer cells for in vitro studies. In this work, C643 ATC cell line was cultured on polymeric scaffolds with high-interconnecte…

0301 basic medicinePolymersBiophysicsMalignancyStem cell markerThyroid Carcinoma AnaplasticBiochemistryMetastasis03 medical and health sciences0302 clinical medicineCancer stem cellCell Line TumormedicineBiomarkers TumorHumansDoxorubicin3D tumor model Anaplastic thyroid carcinoma Doxorubicin Polymeric scaffold Stem cell markersMolecular BiologyThyroid cancerCell ShapeCell ProliferationTissue Scaffoldsbusiness.industryThyroidCell Biologymedicine.disease030104 developmental biologymedicine.anatomical_structureDoxorubicin030220 oncology & carcinogenesisCancer cellCancer researchDisease ProgressionNeoplastic Stem Cellsbusinessmedicine.drugBiochemical and biophysical research communications
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PLLA scaffolds with controlled architecture as potential microenvironment for in vitro tumor model

2019

Abstract The "microenvironment" where a tumor develops plays a fundamental role in determining its progression, the onset of metastasis and, eventually, its resistance to therapies. Tumor cells can be considered more or less invasive depending both on the nature of the cells and on the site where they are located. Commonly adopted laboratory culture protocols for the investigation of tumor cells take usually place on standard two-dimensional supports. However, such cultures do not allow for reproduction of the biophysical properties of the tumor’s microenvironment, thus causing the cells to lose most of their relevant characteristics. In this work MDA-MB 231 breast cancer cells were cultiva…

3D tumor modelPolyestersCellBreast Neoplasms02 engineering and technologyBiologyModels BiologicalMetastasis03 medical and health scienceschemistry.chemical_compoundCell Line TumorTumor MicroenvironmentmedicineHumansViability assayDAPICell adhesion030304 developmental biologySettore ING-IND/24 - Principi Di Ingegneria Chimica0303 health sciencesTissue ScaffoldsSettore ING-IND/34 - Bioingegneria IndustrialeBreast cancer cellCell BiologyGeneral Medicine021001 nanoscience & nanotechnologymedicine.diseaseIn vitroLactic acidStainingmedicine.anatomical_structurechemistryBiophysicsFemaleThermally induced phase separation0210 nano-technologyPoly-L-Lactic acidDevelopmental BiologyTissue and Cell
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